Imaging-Based Screening Platform Assists Protein Engineering
نویسندگان
چکیده
منابع مشابه
In vitro flow cytometry-based screening platform for cellulase engineering
Ultrahigh throughput screening (uHTS) plays an essential role in directed evolution for tailoring biocatalysts for industrial applications. Flow cytometry-based uHTS provides an efficient coverage of the generated protein sequence space by analysis of up to 10(7) events per hour. Cell-free enzyme production overcomes the challenge of diversity loss during the transformation of mutant libraries ...
متن کاملBxb1 phage recombinase assists genome engineering in Drosophila melanogaster.
Rapid and reliable genome modifications provide the basis for detailed in vivo functional analysis of any genomic entity (gene, regulatory DNA, non-coding RNA, etc). With the advent of CRISPR/Cas9 genome editing technology, manipulation of a particular genomic locus has become a routine undertaking in variety of model organisms, including the fruit fly Drosophila melanogaster. To further divers...
متن کاملA complete microfluidic screening platform for rational protein crystallization.
By virtue of their unique mass transport properties, dense integration, and low reagent consumption, microfluidic devices have emerged as viable technologies for protein crystallization. Current microfluidic crystallization technologies have focused on two separate strategies: one using the large-scale integration of active microvalves,1 and one using two-phase flows for the mixing and encapsul...
متن کاملNanocomposite Based on Albumin Protein and Review of Its Applications in Medical Engineering
Albumin is a protein macromolecule that it has been attractive since the early 20th century and has several therapeutic applications. Albumin is an aboundant serum with good biocompatibility, hematopoiesis and biodegradability in blood. The existence of multiple functional groups makes it possible to carry significant amounts of therapeutic and diagnostic factors, as well as targeting factors. ...
متن کاملLaser-based imaging for reverse engineering
In this paper, we explore the technical challenges to automatically generate computer-aided design models of existing vehicle parts using laser range imaging techniques. We propose a complete system that integrates data acquisition and model reconstruction. We discuss methods to resolve the occlusion problem and the associated registration problem. We also present our reconstruction algorithm. ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cell Chemical Biology
سال: 2018
ISSN: 2451-9456
DOI: 10.1016/j.chembiol.2018.08.008